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Backgrounds: Justicia gendarussa Burm.f. Has been known to have anti-HIV activity. This study was conducted to evaluate the effect of incubation time on the antiviral activity of the J.
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Gendarussa leaves extract on HIV-infected MT-4 cells in vitro. Molecular docking test was also conducted to determine the interaction of alkaloids and flavonoids on the J. Gendarussa leaves against HIV-1 reverse transcriptase receptor. It is expected that this research will provide scientific information on the development of J. Gendarussa leaves as an anti-HIV drug. Materials and Methods: In the activity test, the effect of incubation time on the antiviral activity of J.
Gendarussa leaves on HIV-infected MT-4 cells were evaluated. During the activity test, a parameter of cytolysis effect inhibition on MT-4 cell line was observed after 4 days and 6 days incubation period. The molecular docking test is performed by using Molegro Virtual Docker software to determine the interaction of alkaloid and flavonoid compounds of J. Gendarussa leaves with HIV-1 reverse transcriptase receptor. Introduction Human Immunodeficiency Virus (HIV) is a retrovirus that infects the human immune system. HIV damages the human immune system by destroying T lymphocyte cell (a type of leukocyte).